Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 115247..115890 Replicon plasmid unnamed2
Accession NZ_CP069572
Organism Escherichia coli strain FDAARGOS_1250

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag I6J80_RS24580 Protein ID WP_001044768.1
Coordinates 115474..115890 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag I6J80_RS24575 Protein ID WP_001261287.1
Coordinates 115247..115477 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J80_RS24555 110407..111495 + 1089 WP_000952217.1 transcriptional repressor PifC -
I6J80_RS24560 111497..113722 + 2226 WP_000698737.1 phage T7 exclusion protein -
I6J80_RS24565 113772..114671 - 900 WP_000963206.1 nucleotide-binding protein -
I6J80_RS24570 114661..114951 - 291 WP_000111771.1 hypothetical protein -
I6J80_RS24575 115247..115477 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I6J80_RS24580 115474..115890 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
I6J80_RS24585 116052..118190 - 2139 WP_000350638.1 AAA family ATPase -
I6J80_RS24590 118544..118801 + 258 WP_000343085.1 hypothetical protein -
I6J80_RS24595 118801..119391 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / ant(3'')-Ia / sul3 / dfrA14 / aph(3')-Ia / aac(3)-IIa / sitABCD / blaCTX-M-55 / tet(A) iutA / iucD / iucC / iucB / iucA 1..135042 135042


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T190992 WP_001044768.1 NZ_CP069572:115474-115890 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T190992 NZ_CP091607:c4043283-4042999 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGACGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT190992 WP_001261287.1 NZ_CP069572:115247-115477 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT190992 NZ_CP091607:c4043515-4043273 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References