Detailed information of TA system TA190987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 39273..39898
• Replicon: plasmid unnamed2
• Accession: NZ_CP069572
• Organism: Escherichia coli strain FDAARGOS_1250

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6J80_RS24130
• Protein ID: WP_000911313.1
• Coordinates: 39500..39898 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: I6J80_RS24125
• Protein ID: WP_000450520.1
• Coordinates: 39273..39500 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J80_RS24125	39273..39500	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
I6J80_RS24130	39500..39898	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I6J80_RS24135	39907..42060	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
I6J80_RS24140	42313..43044	-	732	WP_023565183.1	complement resistance protein TraT	-
I6J80_RS24145	43076..43573	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / ant(3'')-Ia / sul3 / dfrA14 / aph(3')-Ia / aac(3)-IIa / sitABCD / blaCTX-M-55 / tet(A)	iutA / iucD / iucC / iucB / iucA	1..135042	135042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T190987 WP_000911313.1 NZ_CP069572:39500-39898 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T190987 NZ_CP091607:845390-845803 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CGCGCCAACACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT190987 WP_000450520.1 NZ_CP069572:39273-39500 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT190987 NZ_CP091607:845143-845409 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2