Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2017332..2017986 Replicon chromosome
Accession NZ_CP069571
Organism Escherichia coli strain FDAARGOS_1250

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag I6J80_RS10220 Protein ID WP_000244783.1
Coordinates 2017579..2017986 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6J80_RS10215 Protein ID WP_000354046.1
Coordinates 2017332..2017598 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J80_RS10190 2012501..2013244 + 744 WP_000951948.1 SDR family oxidoreductase -
I6J80_RS10195 2013301..2014734 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
I6J80_RS10200 2014779..2015090 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
I6J80_RS10205 2015254..2015913 + 660 WP_000250274.1 hemolysin III family protein -
I6J80_RS10210 2016109..2017089 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
I6J80_RS10215 2017332..2017598 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6J80_RS10220 2017579..2017986 + 408 WP_000244783.1 protein YgfX Toxin
I6J80_RS10225 2018026..2018547 - 522 WP_001055874.1 flavodoxin FldB -
I6J80_RS10230 2018659..2019555 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6J80_RS10235 2019580..2020290 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6J80_RS10240 2020296..2022029 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T190968 WP_000244783.1 NZ_CP069571:2017579-2017986 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T190968 NZ_CP091601:1866970-1867287 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT190968 WP_000354046.1 NZ_CP069571:2017332-2017598 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT190968 NZ_CP091601:1867280-1867567 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References