Detailed information of TA system TA190964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 392820..393634
• Replicon: chromosome
• Accession: NZ_CP069571
• Organism: Escherichia coli strain FDAARGOS_1250

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: I6J80_RS02405
• Protein ID: WP_001054376.1
• Coordinates: 392820..393077 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: I6J80_RS02410
• Protein ID: WP_001309181.1
• Coordinates: 393089..393634 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J80_RS02385	388108..389214	+	1107	WP_023568191.1	N-acetylneuraminate epimerase	-
I6J80_RS02390	389279..390259	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
I6J80_RS02395	390842..392082	-	1241	Protein_363	DNA helicase	-
I6J80_RS02400	392198..392443	+	246	Protein_364	GNAT family N-acetyltransferase	-
I6J80_RS02405	392820..393077	+	258	WP_001054376.1	hypothetical protein	Toxin
I6J80_RS02410	393089..393634	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
I6J80_RS02415	393690..394436	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
I6J80_RS02420	394605..394823	+	219	Protein_368	hypothetical protein	-
I6J80_RS02425	394861..394977	+	117	Protein_369	VOC family protein	-
I6J80_RS02430	395222..396343	+	1122	WP_000010829.1	M42 family metallopeptidase	-
I6J80_RS02435	396340..396618	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
I6J80_RS02440	396630..397943	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	382606..401858	19252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T190964 WP_001054376.1 NZ_CP069571:392820-393077 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T190964 NZ_CP091601:569255-569638 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT190964 WP_001309181.1 NZ_CP069571:393089-393634 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT190964 NZ_CP091601:569013-569255 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8