Detailed information of TA system TA190960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 17628..18229
• Replicon: plasmid unnamed1
• Accession: NZ_CP069570
• Organism: Escherichia coli strain FDAARGOS_1250

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: I6J80_RS00085
• Protein ID: WP_001216045.1
• Coordinates: 17628..18008 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: I6J80_RS00090
• Protein ID: WP_001190712.1
• Coordinates: 18008..18229 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J80_RS00060	13069..14553	-	1485	WP_000124159.1	hypothetical protein	-
I6J80_RS00065	14553..15746	-	1194	WP_000219618.1	hypothetical protein	-
I6J80_RS00070	15832..16284	-	453	WP_001326849.1	late promoter-activating protein	-
I6J80_RS00075	16373..17416	-	1044	WP_000648833.1	DUF968 domain-containing protein	-
I6J80_RS00080	17444..17623	-	180	WP_074465253.1	PdcA protein	-
I6J80_RS00085	17628..18008	-	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
I6J80_RS00090	18008..18229	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6J80_RS00095	18302..18691	-	390	WP_204156867.1	DNA repair protein	-
I6J80_RS00100	18815..19066	-	252	WP_032153798.1	DNA polymerase III subunit theta	-
I6J80_RS00105	19381..19641	-	261	WP_141085812.1	hypothetical protein	-
I6J80_RS00110	19646..19930	-	285	WP_094506073.1	antitoxin PHD	-
I6J80_RS00115	19914..20393	-	480	WP_204156872.1	hypothetical protein	-
I6J80_RS00120	21215..21424	-	210	WP_000951710.1	hypothetical protein	-
I6J80_RS00125	21426..21614	-	189	WP_204156868.1	hypothetical protein	-
I6J80_RS00130	21787..22233	-	447	WP_204156869.1	ead/Ea22-like family protein	-
I6J80_RS00135	22230..22817	-	588	WP_047647124.1	ead/Ea22-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90593	90593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T190960 WP_001216045.1 NZ_CP069570:c18008-17628 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T190960 NZ_CP091596:2603096-2603485 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAGTAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGTAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT190960 WP_001190712.1 NZ_CP069570:c18229-18008 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT190960 NZ_CP091596:2602832-2603089 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCATTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6