Detailed information of TA system TA190948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1288257..1288786
• Replicon: chromosome
• Accession: NZ_CP069565
• Organism: Staphylococcus aureus strain FDAARGOS_1245

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I6J75_RS06275
• Protein ID: WP_000621175.1
• Coordinates: 1288424..1288786 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: I6J75_RS06270
• Protein ID: WP_000948331.1
• Coordinates: 1288257..1288427 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J75_RS06240	1283294..1283854	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
I6J75_RS06245	1284062..1284541	+	480	WP_001287088.1	hypothetical protein	-
I6J75_RS06250	1284534..1286117	+	1584	WP_001294626.1	PH domain-containing protein	-
I6J75_RS06255	1286104..1286595	+	492	WP_001205910.1	PH domain-containing protein	-
I6J75_RS06260	1286599..1286958	+	360	WP_000581200.1	holo-ACP synthase	-
I6J75_RS06265	1287024..1288172	+	1149	WP_001281145.1	alanine racemase	-
I6J75_RS06270	1288257..1288427	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
I6J75_RS06275	1288424..1288786	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6J75_RS06280	1289135..1290136	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
I6J75_RS06285	1290255..1290581	+	327	WP_001052491.1	anti-sigma factor antagonist	-
I6J75_RS06290	1290583..1291062	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
I6J75_RS06295	1291037..1291807	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T190948 WP_000621175.1 NZ_CP069565:1288424-1288786 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T190948 NZ_CP091594:c389219-388686 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT190948 WP_000948331.1 NZ_CP069565:1288257-1288427 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT190948 NZ_CP091594:c389496-389230 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1