Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 4328..4929 Replicon plasmid unnamed1
Accession NZ_CP069560
Organism Escherichia coli strain FDAARGOS_1259

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag I6J89_RS24230 Protein ID WP_001216034.1
Coordinates 4549..4929 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag I6J89_RS24225 Protein ID WP_001190712.1
Coordinates 4328..4549 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J89_RS24215 1309..2592 - 1284 WP_001617890.1 restriction endonuclease subunit S -
I6J89_RS24220 2589..4145 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
I6J89_RS24225 4328..4549 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I6J89_RS24230 4549..4929 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I6J89_RS24235 4934..5113 + 180 WP_001513661.1 hypothetical protein -
I6J89_RS24240 5141..5500 + 360 WP_001513660.1 hypothetical protein -
I6J89_RS24245 5424..5837 + 414 Protein_7 DDE-type integrase/transposase/recombinase -
I6J89_RS24250 5787..6104 - 318 WP_001513659.1 hypothetical protein -
I6J89_RS24255 6332..7348 - 1017 WP_012372828.1 IS5-like element IS5 family transposase -
I6J89_RS24260 7556..8959 + 1404 WP_001373486.1 S-methylmethionine permease -
I6J89_RS24265 8946..9878 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A - 1..67959 67959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T190925 WP_001216034.1 NZ_CP069560:4549-4929 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T190925 NZ_CP091575:8390-8644 [Salmonella enterica]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT190925 WP_001190712.1 NZ_CP069560:4328-4549 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT190925 NZ_CP091575:8641-8883 [Salmonella enterica]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References