Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4851868..4852365 Replicon chromosome
Accession NZ_CP069559
Organism Escherichia coli strain FDAARGOS_1259

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6J89_RS23515 Protein ID WP_001173343.1
Coordinates 4851868..4852041 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6J89_RS23520 Protein ID WP_001312331.1
Coordinates 4852069..4852365 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J89_RS23505 4848538..4849995 + 1458 WP_000856828.1 dipeptide/tripeptide permease DtpC -
I6J89_RS23510 4850232..4851749 + 1518 WP_000003805.1 lysine--tRNA ligase -
I6J89_RS23515 4851868..4852041 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6J89_RS23520 4852069..4852365 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6J89_RS23525 4852594..4852866 - 273 WP_000405647.1 N-acetyltransferase -
I6J89_RS23530 4852878..4853108 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
I6J89_RS23535 4853289..4854920 + 1632 WP_001216466.1 sensor histidine kinase -
I6J89_RS23540 4854917..4855636 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4827990..4852365 24375


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T190924 WP_001173343.1 NZ_CP069559:c4852041-4851868 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T190924 NZ_CP091574:77629-77778 [Salmonella enterica]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT190924 WP_001312331.1 NZ_CP069559:c4852365-4852069 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT190924 NZ_CP091574:c77584-77525 [Salmonella enterica]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References