Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3962039..3962657 Replicon chromosome
Accession NZ_CP069559
Organism Escherichia coli strain FDAARGOS_1259

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6J89_RS19180 Protein ID WP_001291435.1
Coordinates 3962439..3962657 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6J89_RS19175 Protein ID WP_000344800.1
Coordinates 3962039..3962413 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J89_RS19165 3957129..3958322 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6J89_RS19170 3958345..3961494 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
I6J89_RS19175 3962039..3962413 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6J89_RS19180 3962439..3962657 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6J89_RS19185 3962830..3963381 + 552 WP_000102539.1 maltose O-acetyltransferase -
I6J89_RS19190 3963497..3963967 + 471 WP_000136192.1 YlaC family protein -
I6J89_RS19195 3964131..3965681 + 1551 WP_001468081.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6J89_RS19200 3965723..3966076 - 354 WP_000878138.1 DUF1428 family protein -
I6J89_RS19210 3966455..3966766 + 312 WP_000409908.1 MGMT family protein -
I6J89_RS19215 3966797..3967369 - 573 WP_000779844.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T190915 WP_001291435.1 NZ_CP069559:3962439-3962657 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T190915 NZ_CP091571:3526924-3527142 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT190915 WP_000344800.1 NZ_CP069559:3962039-3962413 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT190915 NZ_CP091571:3526522-3526896 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References