Detailed information of TA system TA190912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2838934..2840522
• Replicon: chromosome
• Accession: NZ_CP069559
• Organism: Escherichia coli strain FDAARGOS_1259

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: I6J89_RS13605
• Protein ID: WP_001125475.1
• Coordinates: 2839200..2840522 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6J89_RS13600
• Protein ID: WP_001332145.1
• Coordinates: 2838934..2839200 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J89_RS13585	2835302..2836753	+	1452	WP_000854636.1	tagaturonate reductase	-
I6J89_RS13590	2836950..2837864	+	915	WP_001306069.1	bestrophin family protein	-
I6J89_RS13595	2837868..2838626	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
I6J89_RS13600	2838934..2839200	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6J89_RS13605	2839200..2840522	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6J89_RS13610	2840736..2841053	-	318	WP_045233010.1	hypothetical protein	-
I6J89_RS13615	2841294..2841464	-	171	Protein_2647	transcriptional regulator	-
I6J89_RS13620	2841715..2842425	+	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-
I6J89_RS13625	2842467..2845118	+	2652	WP_059342349.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T190912 WP_001125475.1 NZ_CP069559:2839200-2840522 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T190912 NZ_CP091571:c1028507-1027980 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT190912 WP_001332145.1 NZ_CP069559:2838934-2839200 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT190912 NZ_CP091571:c1028794-1028504 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8