Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2557130..2557680 Replicon chromosome
Accession NZ_CP069549
Organism Escherichia coli strain FDAARGOS_1253

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6J83_RS12320 Protein ID WP_001160966.1
Coordinates 2557130..2557444 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag I6J83_RS12325 Protein ID WP_000125566.1
Coordinates 2557447..2557680 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J83_RS12295 2552805..2554091 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
I6J83_RS12300 2554091..2555080 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6J83_RS12305 2555077..2555898 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6J83_RS12310 2555901..2556278 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6J83_RS12315 2556285..2557133 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6J83_RS12320 2557130..2557444 - 315 WP_001160966.1 CcdB family protein Toxin
I6J83_RS12325 2557447..2557680 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6J83_RS12330 2557766..2558245 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6J83_RS12335 2558437..2560299 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
I6J83_RS12340 2560292..2560822 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6J83_RS12345 2560930..2562261 - 1332 WP_001183207.1 MFS transporter -
I6J83_RS12350 2562320..2562607 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T190881 WP_001160966.1 NZ_CP069549:c2557444-2557130 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T190881 NZ_CP091566:1437269-1437580 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT190881 WP_000125566.1 NZ_CP069549:c2557680-2557447 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT190881 NZ_CP091566:1437581-1437871 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References