Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 601857..602447 Replicon chromosome
Accession NZ_CP069549
Organism Escherichia coli strain FDAARGOS_1253

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag I6J83_RS02695 Protein ID WP_000373724.1
Coordinates 602115..602447 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag I6J83_RS02690 Protein ID WP_000288812.1
Coordinates 601857..602114 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J83_RS02670 599470..600606 + 1137 WP_000420971.1 ISAs1 family transposase -
I6J83_RS02675 600839..601306 + 468 WP_000201267.1 hypothetical protein -
I6J83_RS02680 601303..601509 + 207 WP_000983730.1 helix-turn-helix domain-containing protein -
I6J83_RS02685 601568..601759 - 192 WP_077561361.1 hypothetical protein -
I6J83_RS02690 601857..602114 + 258 WP_000288812.1 antitoxin Antitoxin
I6J83_RS02695 602115..602447 + 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I6J83_RS02700 602494..602850 - 357 WP_001147230.1 hypothetical protein -
I6J83_RS02705 602831..603157 - 327 Protein_532 hypothetical protein -
I6J83_RS02710 603295..604173 + 879 WP_000511773.1 integrase domain-containing protein -
I6J83_RS02715 605125..605583 + 459 WP_000526101.1 IS200/IS605 family transposase -
I6J83_RS02720 605722..606984 - 1263 WP_000055690.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 572723..617949 45226
- inside IScluster/Tn - - 599470..605583 6113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T190867 WP_000373724.1 NZ_CP069549:602115-602447 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T190867 NZ_CP091563:c4477039-4476671 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT190867 WP_000288812.1 NZ_CP069549:601857-602114 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT190867 NZ_CP091563:c4477257-4477036 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References