Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 19318..19853 Replicon plasmid unnamed2
Accession NZ_CP069544
Organism Escherichia coli strain FDAARGOS_1248

Toxin (Protein)


Gene name relE Uniprot ID T2HS21
Locus tag I6J78_RS24110 Protein ID WP_000222775.1
Coordinates 19566..19853 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T2HSA2
Locus tag I6J78_RS24105 Protein ID WP_001139207.1
Coordinates 19318..19569 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J78_RS24075 15810..16498 - 689 Protein_14 IS3 family transposase -
I6J78_RS24080 16522..16668 + 147 WP_072007499.1 type I toxin-antitoxin system Hok family toxin -
I6J78_RS24085 16954..17214 + 261 WP_032291144.1 replication regulatory protein RepA -
I6J78_RS24090 17440..17514 + 75 WP_001365705.1 RepA leader peptide Tap -
I6J78_RS24095 17507..18364 + 858 WP_032291142.1 incFII family plasmid replication initiator RepA -
I6J78_RS24100 19064..19231 + 168 WP_164722579.1 hypothetical protein -
I6J78_RS24105 19318..19569 + 252 WP_001139207.1 plasmid stabilization protein Antitoxin
I6J78_RS24110 19566..19853 + 288 WP_000222775.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6J78_RS24115 19863..20407 + 545 Protein_22 hypothetical protein -
I6J78_RS24120 20925..21314 + 390 WP_204148185.1 transposase -
I6J78_RS24125 21311..21658 + 348 WP_175091151.1 IS66 family insertion sequence element accessory protein TnpB -
I6J78_RS24130 21707..23245 + 1539 WP_175091152.1 IS66-like element ISEc22 family transposase -
I6J78_RS24135 23583..24172 + 590 Protein_26 serine acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - espC 1..80965 80965
- inside IScluster/Tn - - 15744..23245 7501


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.17 Da        Isoelectric Point: 10.6261

>T190865 WP_000222775.1 NZ_CP069544:19566-19853 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

>T190865 NZ_CP091563:c3874446-3874033 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9216.45 Da        Isoelectric Point: 4.0927

>AT190865 WP_001139207.1 NZ_CP069544:19318-19569 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT190865 NZ_CP091563:c3874693-3874427 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB T2HS21

Antitoxin

Source ID Structure
AlphaFold DB A0A754EQ65

References