Detailed information of TA system TA190862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4641289..4642972
• Replicon: chromosome
• Accession: NZ_CP069542
• Organism: Escherichia coli strain FDAARGOS_1248

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: I2UPR9
• Locus tag: I6J78_RS22295
• Protein ID: WP_000361917.1
• Coordinates: 4642055..4642972 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: I6J78_RS22290
• Protein ID: WP_000157137.1
• Coordinates: 4641289..4642062 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J78_RS22275	4637158..4639242	+	2085	WP_050939399.1	protease Lon-related BREX system protein BrxL	-
I6J78_RS22280	4639361..4640710	+	1350	WP_000766442.1	phospholipase	-
I6J78_RS22285	4640885..4641205	-	321	Protein_4339	IS3 family transposase	-
I6J78_RS22290	4641289..4642062	+	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
I6J78_RS22295	4642055..4642972	+	918	WP_000361917.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
I6J78_RS22300	4643266..4643433	+	168	WP_001356018.1	hypothetical protein	-
I6J78_RS22305	4643486..4644148	-	663	WP_000357928.1	hypothetical protein	-
I6J78_RS22310	4644152..4644862	-	711	WP_001219054.1	integrase arm-type DNA-binding domain-containing protein	-
I6J78_RS22315	4645326..4645478	-	153	WP_001280445.1	hypothetical protein	-
I6J78_RS22320	4645563..4645760	-	198	WP_000839281.1	DUF957 domain-containing protein	-
I6J78_RS22325	4645772..4646260	-	489	WP_000777547.1	hypothetical protein	-
I6J78_RS22330	4646257..4646631	-	375	WP_000854753.1	TA system toxin CbtA family protein	-
I6J78_RS22335	4646721..4647089	-	369	WP_204148142.1	type IV toxin-antitoxin system YeeU family antitoxin	-
I6J78_RS22340	4647252..4647473	-	222	WP_000692345.1	DUF987 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34963.04 Da
• Isoelectric Point: 4.7716

>T190862 WP_000361917.1 NZ_CP069542:4642055-4642972 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELEAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T190862 NZ_CP091563:c2631330-2631227 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT190862 WP_000157137.1 NZ_CP069542:4641289-4642062 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT190862 NZ_CP091563:2631225-2631370 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Structures

Toxin

• AlphaFold DB: A0A0H0PS08

Antitoxin

• AlphaFold DB: A0A0H0N3W2