Detailed information of TA system TA190858

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4198665..4199359
• Replicon: chromosome
• Accession: NZ_CP069542
• Organism: Escherichia coli strain FDAARGOS_1248

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A2A3UTQ9
• Locus tag: I6J78_RS20240
• Protein ID: WP_001094399.1
• Coordinates: 4198665..4199033 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: I6J78_RS20245
• Protein ID: WP_077249034.1
• Coordinates: 4199054..4199359 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J78_RS20220	4193838..4194794	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
I6J78_RS20225	4194791..4196989	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
I6J78_RS20230	4196999..4197955	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
I6J78_RS20235	4198006..4198344	+	339	Protein_3940	LysR family transcriptional regulator	-
I6J78_RS20240	4198665..4199033	-	369	WP_001094399.1	TA system toxin CbtA family protein	Toxin
I6J78_RS20245	4199054..4199359	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J78_RS20250	4199463..4200107	-	645	WP_000086755.1	hypothetical protein	-
I6J78_RS20255	4200126..4200347	-	222	WP_000692307.1	DUF987 domain-containing protein	-
I6J78_RS20260	4200416..4200892	-	477	WP_001573263.1	RadC family protein	-
I6J78_RS20265	4200908..4201381	-	474	WP_114420036.1	antirestriction protein	-
I6J78_RS20270	4201475..4201720	-	246	WP_000680583.1	hypothetical protein	-
I6J78_RS20275	4201720..4202538	-	819	WP_001573260.1	DUF945 domain-containing protein	-
I6J78_RS20280	4202638..4202871	-	234	WP_001119729.1	DUF905 family protein	-
I6J78_RS20285	4202946..4203401	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagW/ecpD / yagV/ecpE	4173256..4200892	27636

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13568.73 Da
• Isoelectric Point: 7.7465

>T190858 WP_001094399.1 NZ_CP069542:c4199033-4198665 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T190858 NZ_CP091563:583700-583987 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT190858 WP_077249034.1 NZ_CP069542:c4199359-4199054 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT190858 NZ_CP091563:583974-584276 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A2A3UTQ9