Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4015115..4015733 Replicon chromosome
Accession NZ_CP069542
Organism Escherichia coli strain FDAARGOS_1248

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6J78_RS19380 Protein ID WP_001291435.1
Coordinates 4015515..4015733 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6J78_RS19375 Protein ID WP_000344800.1
Coordinates 4015115..4015489 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J78_RS19365 4010204..4011397 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6J78_RS19370 4011420..4014569 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
I6J78_RS19375 4015115..4015489 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6J78_RS19380 4015515..4015733 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6J78_RS19385 4015905..4016456 + 552 WP_000102564.1 maltose O-acetyltransferase -
I6J78_RS19390 4016572..4017042 + 471 WP_000136192.1 YlaC family protein -
I6J78_RS19395 4017206..4018756 + 1551 WP_158137990.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6J78_RS19400 4018798..4019151 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6J78_RS19410 4019530..4019841 + 312 WP_000409911.1 MGMT family protein -
I6J78_RS19415 4019872..4020444 - 573 WP_000779838.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T190857 WP_001291435.1 NZ_CP069542:4015515-4015733 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T190857 NZ_CP091563:489731-490015 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT190857 WP_000344800.1 NZ_CP069542:4015115-4015489 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT190857 NZ_CP091563:489499-489741 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References