Detailed information of TA system TA190832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 27178..27737
• Replicon: plasmid unnamed2
• Accession: NZ_CP069530
• Organism: Escherichia coli strain FDAARGOS_1252

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A376ZSB3
• Locus tag: I6J82_RS25505
• Protein ID: WP_021515398.1
• Coordinates: 27462..27737 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6J82_RS25500
• Protein ID: WP_024187480.1
• Coordinates: 27178..27462 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J82_RS25460	22654..22914	+	261	WP_097403166.1	hypothetical protein	-
I6J82_RS25465	23021..23284	+	264	WP_001089474.1	hypothetical protein	-
I6J82_RS25470	23274..23537	+	264	Protein_24	type II toxin-antitoxin system RelE/ParE family toxin	-
I6J82_RS25475	23618..25156	-	1539	WP_016245515.1	IS66 family transposase	-
I6J82_RS25480	25206..25553	-	348	WP_151333331.1	IS66 family insertion sequence element accessory protein TnpB	-
I6J82_RS25485	25550..25939	-	390	WP_001282151.1	IS66 family insertion sequence hypothetical protein	-
I6J82_RS25490	26024..26122	+	99	Protein_28	entry exclusion protein 1	-
I6J82_RS25495	26203..26631	+	429	WP_097403215.1	Exc2 family lipoprotein	-
I6J82_RS25500	27178..27462	+	285	WP_024187480.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6J82_RS25505	27462..27737	+	276	WP_021515398.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J82_RS25510	28003..28497	-	495	Protein_32	transposase	-
I6J82_RS25515	28774..29649	+	876	WP_123004305.1	ChaN family lipoprotein	-
I6J82_RS25520	29657..30433	+	777	WP_097403217.1	energy transducer TonB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id	senB / iroN / iroE / iroD / iroC / iroB	1..96693	96693
	-	inside	IScluster/Tn	-	-	23618..28497	4879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10778.55 Da
• Isoelectric Point: 10.2344

>T190832 WP_021515398.1 NZ_CP069530:27462-27737 [Escherichia coli]
MELKWTSKALSNLARLYDFLVLASKPAAARMVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T190832 NZ_CP091558:3722091-3722489 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10470.89 Da
• Isoelectric Point: 5.6558

>AT190832 WP_024187480.1 NZ_CP069530:27178-27462 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT190832 NZ_CP091558:3721864-3722091 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A0A376ZSB3