Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3321264..3321814 Replicon chromosome
Accession NZ_CP069528
Organism Escherichia coli strain FDAARGOS_1252

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6J82_RS15945 Protein ID WP_001160966.1
Coordinates 3321264..3321578 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag I6J82_RS15950 Protein ID WP_000125568.1
Coordinates 3321581..3321814 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J82_RS15920 3316939..3318225 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
I6J82_RS15925 3318225..3319214 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6J82_RS15930 3319211..3320032 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6J82_RS15935 3320035..3320412 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6J82_RS15940 3320419..3321267 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6J82_RS15945 3321264..3321578 - 315 WP_001160966.1 CcdB family protein Toxin
I6J82_RS15950 3321581..3321814 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6J82_RS15955 3321900..3322379 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6J82_RS15960 3322571..3324433 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
I6J82_RS15965 3324426..3324956 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6J82_RS15970 3325064..3326395 - 1332 WP_001183207.1 MFS transporter -
I6J82_RS15975 3326454..3326741 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T190809 WP_001160966.1 NZ_CP069528:c3321578-3321264 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T190809 NZ_CP091556:316725-317093 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT190809 WP_000125568.1 NZ_CP069528:c3321814-3321581 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT190809 NZ_CP091556:316507-316728 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References