Detailed information of TA system TA190782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4313953..4314529
• Replicon: chromosome
• Accession: NZ_CP069522
• Organism: Escherichia coli strain FDAARGOS_1267

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A0G9FRD2
• Locus tag: I6J97_RS20880
• Protein ID: WP_001350372.1
• Coordinates: 4314242..4314529 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0G9FLJ7
• Locus tag: I6J97_RS20875
• Protein ID: WP_000063155.1
• Coordinates: 4313953..4314255 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J97_RS20860	4310481..4312631	+	2151	WP_001314407.1	pyruvate/proton symporter BtsT	-
I6J97_RS20865	4312749..4312952	+	204	WP_000467859.1	YbdD/YjiX family protein	-
I6J97_RS20870	4312963..4313919	+	957	WP_001350371.1	GTPase	-
I6J97_RS20875	4313953..4314255	-	303	WP_000063155.1	BrnA antitoxin family protein	Antitoxin
I6J97_RS20880	4314242..4314529	-	288	WP_001350372.1	BrnT family toxin	Toxin
I6J97_RS20885	4314726..4315892	+	1167	WP_000800824.1	restriction endonuclease	-
I6J97_RS20890	4315956..4317575	+	1620	WP_001029741.1	type I restriction-modification system subunit M	-
I6J97_RS20895	4317565..4318896	+	1332	WP_000505211.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11209.68 Da
• Isoelectric Point: 7.4020

>T190782 WP_001350372.1 NZ_CP069522:c4314529-4314242 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T190782 NZ_CP091547:c2632679-2632576 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11572.05 Da
• Isoelectric Point: 9.4646

>AT190782 WP_000063155.1 NZ_CP069522:c4314255-4313953 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT190782 NZ_CP091547:2632574-2632719 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Structures

Toxin

• AlphaFold DB: A0A0G9FRD2

Antitoxin

• AlphaFold DB: A0A0G9FLJ7