Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4818094..4818644 Replicon chromosome
Accession NZ_CP069517
Organism Escherichia coli strain FDAARGOS_1262

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6J92_RS23115 Protein ID WP_001160966.1
Coordinates 4818330..4818644 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag I6J92_RS23110 Protein ID WP_000125566.1
Coordinates 4818094..4818327 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J92_RS23085 4813167..4813454 + 288 WP_000203741.1 ferredoxin-like protein FixX -
I6J92_RS23090 4813513..4814844 + 1332 WP_001183207.1 MFS transporter -
I6J92_RS23095 4814952..4815482 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6J92_RS23100 4815475..4817337 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
I6J92_RS23105 4817529..4818008 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6J92_RS23110 4818094..4818327 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6J92_RS23115 4818330..4818644 + 315 WP_001160966.1 CcdB family protein Toxin
I6J92_RS23120 4818641..4819489 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6J92_RS23125 4819496..4819873 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6J92_RS23130 4819876..4820697 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6J92_RS23135 4820694..4821683 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6J92_RS23140 4821683..4822969 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T190757 WP_001160966.1 NZ_CP069517:4818330-4818644 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T190757 NZ_CP091542:c4816227-4815916 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT190757 WP_000125566.1 NZ_CP069517:4818094-4818327 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT190757 NZ_CP091542:c4815915-4815625 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References