Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4343965..4344462 Replicon chromosome
Accession NZ_CP069517
Organism Escherichia coli strain FDAARGOS_1262

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6J92_RS20770 Protein ID WP_001173343.1
Coordinates 4344289..4344462 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6J92_RS20765 Protein ID WP_001312331.1
Coordinates 4343965..4344261 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J92_RS20745 4340694..4341413 - 720 WP_000611288.1 two-component system response regulator DcuR -
I6J92_RS20750 4341410..4343041 - 1632 WP_204148026.1 sensor histidine kinase -
I6J92_RS20755 4343222..4343452 + 231 WP_000373291.1 (4Fe-4S)-binding protein -
I6J92_RS20760 4343464..4343736 + 273 WP_000405647.1 N-acetyltransferase -
I6J92_RS20765 4343965..4344261 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6J92_RS20770 4344289..4344462 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6J92_RS20775 4344581..4346098 - 1518 WP_000003805.1 lysine--tRNA ligase -
I6J92_RS20780 4346335..4347792 - 1458 WP_000856828.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T190752 WP_001173343.1 NZ_CP069517:4344289-4344462 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T190752 NZ_CP091542:3536545-3536763 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT190752 WP_001312331.1 NZ_CP069517:4343965-4344261 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT190752 NZ_CP091542:3536143-3536517 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References