Detailed information of TA system TA190744

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1730492..1731323
• Replicon: chromosome
• Accession: NZ_CP069517
• Organism: Escherichia coli strain FDAARGOS_1262

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: I6J92_RS08390
• Protein ID: WP_000854814.1
• Coordinates: 1730949..1731323 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A4Q4I1U9
• Locus tag: I6J92_RS08385
• Protein ID: WP_001519295.1
• Coordinates: 1730492..1730860 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J92_RS08355	1726691..1727344	+	654	WP_001535423.1	hypothetical protein	-
I6J92_RS08360	1727360..1727770	+	411	WP_000846705.1	hypothetical protein	-
I6J92_RS08365	1727991..1728812	+	822	WP_001234710.1	DUF945 domain-containing protein	-
I6J92_RS08370	1729075..1729548	+	474	WP_000855071.1	antirestriction protein	-
I6J92_RS08375	1729563..1730039	+	477	WP_001534813.1	RadC family protein	-
I6J92_RS08380	1730108..1730329	+	222	WP_000692323.1	DUF987 domain-containing protein	-
I6J92_RS08385	1730492..1730860	+	369	WP_001519295.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J92_RS08390	1730949..1731323	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
I6J92_RS08395	1731320..1731514	+	195	WP_000988601.1	hypothetical protein	-
I6J92_RS08400	1731527..1731640	+	114	WP_001161660.1	DUF957 domain-containing protein	-
I6J92_RS08405	1731929..1732075	-	147	Protein_1647	transposase domain-containing protein	-
I6J92_RS08410	1732129..1732311	+	183	WP_001016348.1	hypothetical protein	-
I6J92_RS08415	1732412..1732741	-	330	WP_000450409.1	DUF496 family protein	-
I6J92_RS08420	1732913..1733971	-	1059	WP_001200894.1	FUSC family protein	-
I6J92_RS08425	1734169..1734642	-	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
I6J92_RS08430	1734761..1735927	-	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T190744 WP_000854814.1 NZ_CP069517:1730949-1731323 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T190744 NZ_CP091542:365052-365327 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13654.51 Da
• Isoelectric Point: 6.7389

>AT190744 WP_001519295.1 NZ_CP069517:1730492-1730860 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDANVYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADILGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT190744 NZ_CP091542:364789-365049 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A4Q4I1U9