Detailed information of TA system TA190733

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 680617..681418
• Replicon: chromosome
• Accession: NZ_CP069517
• Organism: Escherichia coli strain FDAARGOS_1262

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2V687
• Locus tag: I6J92_RS03190
• Protein ID: WP_000854739.1
• Coordinates: 681038..681418 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: I6J92_RS03185
• Protein ID: WP_001285482.1
• Coordinates: 680617..680991 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J92_RS03145	676653..677108	+	456	WP_000581502.1	hypothetical protein	-
I6J92_RS03150	677209..677343	+	135	WP_032144430.1	DUF905 family protein	-
I6J92_RS03155	677498..678316	+	819	WP_001560884.1	DUF945 domain-containing protein	-
I6J92_RS03160	678316..678561	+	246	WP_001164966.1	hypothetical protein	-
I6J92_RS03165	678655..679128	+	474	WP_001313575.1	antirestriction protein	-
I6J92_RS03170	679144..679620	+	477	WP_001313574.1	RadC family protein	-
I6J92_RS03175	679683..679904	+	222	WP_000692315.1	DUF987 domain-containing protein	-
I6J92_RS03180	679923..680567	+	645	WP_000086759.1	hypothetical protein	-
I6J92_RS03185	680617..680991	+	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J92_RS03190	681038..681418	+	381	WP_000854739.1	TA system toxin CbtA family protein	Toxin
I6J92_RS03195	681415..681903	+	489	WP_001518535.1	hypothetical protein	-
I6J92_RS03200	681923..682120	+	198	WP_000772027.1	DUF957 domain-containing protein	-
I6J92_RS03205	682205..683032	+	828	WP_001518536.1	DUF4942 domain-containing protein	-
I6J92_RS03215	683518..684456	+	939	WP_000351277.1	glyoxylate/hydroxypyruvate reductase GhrA	-
I6J92_RS03220	684511..685248	+	738	WP_000283664.1	zinc-binding phosphatase	-
I6J92_RS03225	685272..685826	+	555	WP_001001921.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14214.14 Da
• Isoelectric Point: 6.4773

>T190733 WP_000854739.1 NZ_CP069517:681038-681418 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T190733 NZ_CP091540:2488426-2488710 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT190733 WP_001285482.1 NZ_CP069517:680617-680991 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT190733 NZ_CP091540:2488188-2488436 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A0H2V687

Antitoxin

• AlphaFold DB: A0A0P7R0L9