Detailed information of TA system TA190723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 5815..6403
• Replicon: plasmid unnamed4
• Accession: NZ_CP069508
• Organism: Acinetobacter pittii strain FDAARGOS_1215

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: I6J45_RS18215
• Protein ID: WP_039890878.1
• Coordinates: 5815..6102 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: I6J45_RS18220
• Protein ID: WP_057095489.1
• Coordinates: 6089..6403 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J45_RS18200	3243..4172	+	930	WP_005237331.1	replication initiation protein RepM	-
I6J45_RS18205	4241..4786	+	546	WP_171054282.1	plasmid replication DNA-binding protein	-
I6J45_RS18210	4983..5117	-	135	Protein_3	cysteine hydrolase	-
I6J45_RS18215	5815..6102	+	288	WP_039890878.1	BrnT family toxin	Toxin
I6J45_RS18220	6089..6403	+	315	WP_057095489.1	BrnA antitoxin family protein	Antitoxin
I6J45_RS18225	6626..8764	+	2139	WP_057095490.1	DEAD/DEAH box helicase	-
I6J45_RS18230	8766..9437	+	672	WP_057095488.1	DUF2290 domain-containing protein	-
I6J45_RS18235	9558..10721	-	1164	WP_005237355.1	glutathione-dependent formaldehyde dehydrogenase	-
I6J45_RS18240	10965..11297	-	333	WP_032049750.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11469	11469

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11263.65 Da
• Isoelectric Point: 5.2216

>T190723 WP_039890878.1 NZ_CP069508:5815-6102 [Acinetobacter pittii]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T190723 NZ_CP091535:71410-71808 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12242.87 Da
• Isoelectric Point: 7.3173

>AT190723 WP_057095489.1 NZ_CP069508:6089-6403 [Acinetobacter pittii]
MSMVKYSRKELNEKFSDKQDAEIERLLAKGTVSDDHLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT190723 NZ_CP091535:71183-71410 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA