Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4466904..4467535 Replicon chromosome
Accession NZ_CP069501
Organism Escherichia coli strain FDAARGOS_1268

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag I6J98_RS21320 Protein ID WP_001260301.1
Coordinates 4467260..4467535 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag I6J98_RS21315 Protein ID WP_000593555.1
Coordinates 4466904..4467263 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J98_RS21290 4463037..4463981 + 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -
I6J98_RS21295 4463978..4464811 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
I6J98_RS21300 4464811..4465575 + 765 WP_001136211.1 nickel import ATP-binding protein NikD -
I6J98_RS21305 4465572..4466378 + 807 WP_061092823.1 nickel import ATP-binding protein NikE -
I6J98_RS21310 4466383..4466784 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
I6J98_RS21315 4466904..4467263 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I6J98_RS21320 4467260..4467535 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
I6J98_RS21325 4467608..4468732 - 1125 WP_001314210.1 ABC transporter permease -
I6J98_RS21330 4468732..4471467 - 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
I6J98_RS21335 4471464..4472531 - 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T190711 WP_001260301.1 NZ_CP069501:c4467535-4467260 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T190711 NZ_CP091534:1937221-1937472 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT190711 WP_000593555.1 NZ_CP069501:c4467263-4466904 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT190711 NZ_CP091534:1937469-1937723 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References