Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3836799..3837453 Replicon chromosome
Accession NZ_CP069501
Organism Escherichia coli strain FDAARGOS_1268

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6J98_RS18210 Protein ID WP_000244781.1
Coordinates 3836799..3837206 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6J98_RS18215 Protein ID WP_000354046.1
Coordinates 3837187..3837453 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J98_RS18190 3832756..3834489 - 1734 WP_000813184.1 single-stranded-DNA-specific exonuclease RecJ -
I6J98_RS18195 3834495..3835205 - 711 WP_000715220.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6J98_RS18200 3835230..3836126 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
I6J98_RS18205 3836238..3836759 + 522 WP_001055874.1 flavodoxin FldB -
I6J98_RS18210 3836799..3837206 - 408 WP_000244781.1 protein YgfX Toxin
I6J98_RS18215 3837187..3837453 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6J98_RS18220 3837696..3838676 + 981 WP_000886072.1 tRNA-modifying protein YgfZ -
I6J98_RS18225 3838753..3839412 - 660 WP_000250274.1 hemolysin III family protein -
I6J98_RS18230 3839576..3839887 - 312 WP_001182965.1 N(4)-acetylcytidine aminohydrolase -
I6J98_RS18235 3839932..3841365 + 1434 WP_001361333.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T190709 WP_000244781.1 NZ_CP069501:c3837206-3836799 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T190709 NZ_CP091534:c1257394-1257065 [Escherichia coli]
ATGCAAACTTTATCATTACACCCGACTCGGGCGACTCCGCTCTGTTTGTCACCCGTTGAAACCTGGCAACGGCTACTGGC
TCATCTGTTCTCGCAGCACTATGGCCTGACGCTGAATGACACACCATTCAGTAATGAAACCACCATCCGGGAACATATTG
ATGCCGGAGTATCGCTCAGCGATGCGGTGAACTTTCTGGTAGAAAAATACGGACTTGTTCGTATCGACCGGGAGGGATTT
TCATGGCAAGAGCAAACTCCGTATATTTCCGCAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTGA
CGTGAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT190709 WP_000354046.1 NZ_CP069501:c3837453-3837187 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT190709 NZ_CP091534:c1257798-1257433 [Escherichia coli]
ATGAACAATCACTCCGAATCCGGAACTAAGCCGGAGAATCCCGCCTGCAAGCAGTGGGGACTAAGGTGCACGATCACACC
CTGTTTTGGCGCTCGCCTAGTGCAGGAGGGCAACCGGGTGCATTTTCTCGCTGATCGGGCCGGATTCAACGGTGCTTTCA
GTGACGAGGATGTGTTACGCCTAGACCAAGCTTTTTCACTGATACTCAAGCAACTGGAGCTAATGCTCACCAGCGGCGAG
CTAAATCCCCTCTATCAGCACTGCGTCACGCTTTACCACAACGGGCTCACCTGCGAAGCCGATACACTGGGCTCCTTTGG
TTATGTCTACATTGCTATTTATCCTGACCAGCCTGAACCGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References