Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1124938..1125556 Replicon chromosome
Accession NZ_CP069501
Organism Escherichia coli strain FDAARGOS_1268

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6J98_RS05280 Protein ID WP_001291435.1
Coordinates 1124938..1125156 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6J98_RS05285 Protein ID WP_000344800.1
Coordinates 1125182..1125556 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J98_RS05245 1120227..1120799 + 573 WP_000779831.1 YbaY family lipoprotein -
I6J98_RS05250 1120830..1121141 - 312 WP_000409911.1 MGMT family protein -
I6J98_RS05260 1121520..1121873 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6J98_RS05265 1121915..1123465 - 1551 WP_001361689.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6J98_RS05270 1123629..1124099 - 471 WP_000136192.1 YlaC family protein -
I6J98_RS05275 1124215..1124766 - 552 WP_000102574.1 maltose O-acetyltransferase -
I6J98_RS05280 1124938..1125156 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6J98_RS05285 1125182..1125556 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6J98_RS05290 1126102..1129251 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
I6J98_RS05295 1129274..1130467 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T190695 WP_001291435.1 NZ_CP069501:c1125156-1124938 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T190695 NZ_CP091527:c1222373-1222155 [Klebsiella sp. PCX]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT190695 WP_000344800.1 NZ_CP069501:c1125556-1125182 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT190695 NZ_CP091527:c1222774-1222400 [Klebsiella sp. PCX]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References