Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1090629..1091466 Replicon chromosome
Accession NZ_CP069501
Organism Escherichia coli strain FDAARGOS_1268

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag I6J98_RS05110 Protein ID WP_000227782.1
Coordinates 1090629..1091171 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag I6J98_RS05115 Protein ID WP_001353405.1
Coordinates 1091155..1091466 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J98_RS05085 1085707..1086297 - 591 WP_001276320.1 protein deglycase YajL -
I6J98_RS05090 1086260..1087171 - 912 WP_000705841.1 2-dehydropantoate 2-reductase -
I6J98_RS05095 1087339..1087830 + 492 WP_001138904.1 nucleotide binding protein YajQ -
I6J98_RS05100 1087958..1089322 - 1365 WP_001000978.1 MFS transporter -
I6J98_RS05105 1089729..1090573 + 845 Protein_987 sel1 repeat family protein -
I6J98_RS05110 1090629..1091171 - 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
I6J98_RS05115 1091155..1091466 - 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
I6J98_RS05120 1091651..1092541 - 891 WP_000971327.1 heme o synthase -
I6J98_RS05125 1092553..1092882 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
I6J98_RS05130 1092882..1093496 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
I6J98_RS05135 1093486..1095477 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
I6J98_RS05140 1095499..1096446 - 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T190694 WP_000227782.1 NZ_CP069501:c1091171-1090629 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T190694 NZ_CP091527:512830-513111 [Klebsiella sp. PCX]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAATCTCCAGCAGATTATGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT190694 WP_001353405.1 NZ_CP069501:c1091466-1091155 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT190694 NZ_CP091527:512598-512840 [Klebsiella sp. PCX]
ATGGCCACGCTCAACGTTCGACTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGTCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References