Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 591707..592283 Replicon chromosome
Accession NZ_CP069501
Organism Escherichia coli strain FDAARGOS_1268

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag I6J98_RS02730 Protein ID WP_001131965.1
Coordinates 591707..591994 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag I6J98_RS02735 Protein ID WP_000063158.1
Coordinates 591981..592283 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J98_RS02700 587116..588528 - 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -
I6J98_RS02705 588705..588869 + 165 WP_000394263.1 DUF1127 domain-containing protein -
I6J98_RS02710 588911..589249 - 339 WP_000132633.1 endoribonuclease SymE -
I6J98_RS02715 589464..590132 - 669 Protein_520 DUF3387 domain-containing protein -
I6J98_RS02720 590126..590299 - 174 Protein_521 SAM-dependent DNA methyltransferase -
I6J98_RS02725 590344..591510 - 1167 WP_000800823.1 restriction endonuclease -
I6J98_RS02730 591707..591994 + 288 WP_001131965.1 BrnT family toxin Toxin
I6J98_RS02735 591981..592283 + 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
I6J98_RS02740 592317..593273 - 957 WP_001361361.1 GTPase -
I6J98_RS02745 593284..593487 - 204 WP_000467859.1 YbdD/YjiX family protein -
I6J98_RS02750 593537..595687 - 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
I6J98_RS02755 595955..597175 - 1221 WP_000873756.1 amino acid deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T190693 WP_001131965.1 NZ_CP069501:591707-591994 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T190693 NZ_CP091525:2835836-2836153 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTGATGTGTGATATTGAAGTGATAAAAGAAAAAATCAATGATGTAGCTATCAAACATGG
TTGGTTTGTTGAAGATAAATTTGTCAAAAATGAATTAGAAACAAAACAGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT190693 WP_000063158.1 NZ_CP069501:591981-592283 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT190693 NZ_CP091525:2835624-2835833 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTATCATGAATAATTTTGAAAGTTA
TGAACACACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGATCAGTCCTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAAAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References