Detailed information of TA system TA190691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 485469..486064
• Replicon: chromosome
• Accession: NZ_CP069501
• Organism: Escherichia coli strain FDAARGOS_1268

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: I6J98_RS02255
• Protein ID: WP_000239581.1
• Coordinates: 485714..486064 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: I6J98_RS02250
• Protein ID: WP_001223213.1
• Coordinates: 485469..485720 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J98_RS02240	481134..484913	+	3780	WP_000060873.1	autotransporter assembly complex protein TamB	-
I6J98_RS02245	484916..485257	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
I6J98_RS02250	485469..485720	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
I6J98_RS02255	485714..486064	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
I6J98_RS02260	486143..486673	-	531	WP_000055072.1	inorganic diphosphatase	-
I6J98_RS02265	486983..487939	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
I6J98_RS02270	488072..489574	+	1503	WP_001095661.1	sugar ABC transporter ATP-binding protein	-
I6J98_RS02275	489588..490610	+	1023	WP_001361374.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T190691 WP_000239581.1 NZ_CP069501:485714-486064 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T190691 NZ_CP091525:2762788-2762997 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT190691 WP_001223213.1 NZ_CP069501:485469-485720 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT190691 NZ_CP091525:2762483-2762776 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7