Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 378163..378660 Replicon chromosome
Accession NZ_CP069501
Organism Escherichia coli strain FDAARGOS_1268

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6J98_RS01710 Protein ID WP_001173343.1
Coordinates 378487..378660 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6J98_RS01705 Protein ID WP_000398619.1
Coordinates 378163..378459 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J98_RS01685 374892..375611 - 720 WP_000611288.1 two-component system response regulator DcuR -
I6J98_RS01690 375608..377239 - 1632 WP_061092758.1 sensor histidine kinase -
I6J98_RS01695 377420..377650 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
I6J98_RS01700 377662..377934 + 273 WP_000405647.1 N-acetyltransferase -
I6J98_RS01705 378163..378459 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6J98_RS01710 378487..378660 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6J98_RS01715 378779..380296 - 1518 WP_000003806.1 lysine--tRNA ligase -
I6J98_RS01720 380533..381990 - 1458 WP_061092757.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T190690 WP_001173343.1 NZ_CP069501:378487-378660 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T190690 NZ_CP091525:2703870-2703974 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT190690 WP_000398619.1 NZ_CP069501:378163-378459 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT190690 NZ_CP091525:c2704132-2703969 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAATGCCGGTCAAAGCGAATAGAAGGTT
ATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References