Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 67217..67833 Replicon chromosome
Accession NZ_CP069501
Organism Escherichia coli strain FDAARGOS_1268

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag I6J98_RS00310 Protein ID WP_001129490.1
Coordinates 67459..67833 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag I6J98_RS00305 Protein ID WP_001275523.1
Coordinates 67217..67459 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J98_RS00285 62452..62889 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6J98_RS00290 62934..63875 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
I6J98_RS00295 64291..66105 + 1815 WP_000226324.1 hypothetical protein -
I6J98_RS00300 66779..66997 + 219 WP_001251290.1 ribbon-helix-helix domain-containing protein -
I6J98_RS00305 67217..67459 + 243 WP_001275523.1 ribbon-helix-helix domain-containing protein Antitoxin
I6J98_RS00310 67459..67833 + 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
I6J98_RS00315 67870..68799 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
I6J98_RS00320 68796..69431 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
I6J98_RS00325 69428..70330 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T190689 WP_001129490.1 NZ_CP069501:67459-67833 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T190689 NZ_CP091525:2442787-2443053 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT190689 WP_001275523.1 NZ_CP069501:67217-67459 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT190689 NZ_CP091525:2442536-2442787 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References