Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3852711..3853261 Replicon chromosome
Accession NZ_CP069500
Organism Escherichia coli strain FDAARGOS_1258

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6J88_RS18875 Protein ID WP_001160966.1
Coordinates 3852947..3853261 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag I6J88_RS18870 Protein ID WP_000125566.1
Coordinates 3852711..3852944 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J88_RS18845 3847784..3848071 + 288 WP_000203741.1 ferredoxin-like protein FixX -
I6J88_RS18850 3848130..3849461 + 1332 WP_001183207.1 MFS transporter -
I6J88_RS18855 3849569..3850099 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6J88_RS18860 3850092..3851954 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
I6J88_RS18865 3852146..3852625 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6J88_RS18870 3852711..3852944 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6J88_RS18875 3852947..3853261 + 315 WP_001160966.1 CcdB family protein Toxin
I6J88_RS18880 3853258..3854106 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6J88_RS18885 3854113..3854490 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6J88_RS18890 3854493..3855314 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6J88_RS18895 3855311..3856300 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6J88_RS18900 3856300..3857586 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T190686 WP_001160966.1 NZ_CP069500:3852947-3853261 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T190686 NZ_CP091525:1212433-1212621 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT190686 WP_000125566.1 NZ_CP069500:3852711-3852944 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT190686 NZ_CP091525:1212144-1212404 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References