Detailed information of TA system TA190683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3696818..3697326
• Replicon: chromosome
• Accession: NZ_CP069500
• Organism: Escherichia coli strain FDAARGOS_1258

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: I6J88_RS18030
• Protein ID: WP_001534843.1
• Coordinates: 3696818..3697027 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: I6J88_RS18035
• Protein ID: WP_000063161.1
• Coordinates: 3697024..3697326 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J88_RS18015	3692573..3693883	-	1311	WP_001534837.1	restriction endonuclease subunit S	-
I6J88_RS18020	3693873..3695492	-	1620	WP_016238256.1	type I restriction-modification system subunit M	-
I6J88_RS18025	3695556..3696722	-	1167	WP_001534841.1	restriction endonuclease	-
I6J88_RS18030	3696818..3697027	+	210	WP_001534843.1	BrnT family toxin	Toxin
I6J88_RS18035	3697024..3697326	+	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
I6J88_RS18040	3697360..3698316	-	957	WP_001534845.1	GTPase	-
I6J88_RS18045	3698327..3698530	-	204	WP_001534846.1	YbdD/YjiX family protein	-
I6J88_RS18050	3698648..3700798	-	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T190683 WP_001534843.1 NZ_CP069500:3696818-3697027 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T190683 NZ_CP091525:121774-121926 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT190683 WP_000063161.1 NZ_CP069500:3697024-3697326 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT190683 NZ_CP091525:121150-121749 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3