Detailed information of TA system TA190681

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3327939..3328740
• Replicon: chromosome
• Accession: NZ_CP069500
• Organism: Escherichia coli strain FDAARGOS_1258

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D8EBK0
• Locus tag: I6J88_RS16250
• Protein ID: WP_001094436.1
• Coordinates: 3327939..3328316 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MN76
• Locus tag: I6J88_RS16255
• Protein ID: WP_015953067.1
• Coordinates: 3328363..3328740 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J88_RS16220	3323898..3324059	-	162	Protein_3032	DUF4942 domain-containing protein	-
I6J88_RS16225	3324150..3325649	+	1500	WP_000174402.1	IS21-like element ISEc10 family transposase	-
I6J88_RS16230	3325646..3326401	+	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
I6J88_RS16235	3326465..3327160	-	696	Protein_3035	DUF4942 domain-containing protein	-
I6J88_RS16240	3327245..3327442	-	198	WP_000839254.1	DUF957 domain-containing protein	-
I6J88_RS16245	3327454..3327942	-	489	WP_000761714.1	hypothetical protein	-
I6J88_RS16250	3327939..3328316	-	378	WP_001094436.1	TA system toxin CbtA family protein	Toxin
I6J88_RS16255	3328363..3328740	-	378	WP_015953067.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
I6J88_RS16260	3328819..3329040	-	222	WP_000692312.1	DUF987 domain-containing protein	-
I6J88_RS16265	3329109..3329585	-	477	WP_001186756.1	RadC family protein	-
I6J88_RS16270	3329600..3330085	-	486	WP_000860054.1	antirestriction protein	-
I6J88_RS16275	3330176..3330994	-	819	WP_001175142.1	DUF945 domain-containing protein	-
I6J88_RS16280	3331084..3331317	-	234	WP_001278287.1	DUF905 family protein	-
I6J88_RS16285	3331323..3332000	-	678	WP_001097312.1	hypothetical protein	-
I6J88_RS16290	3332148..3332828	-	681	WP_001282927.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13958.84 Da
• Isoelectric Point: 6.8603

>T190681 WP_001094436.1 NZ_CP069500:c3328316-3327939 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T190681 NZ_CP091523:2755839-2756201 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13766.60 Da
• Isoelectric Point: 6.2021

>AT190681 WP_015953067.1 NZ_CP069500:c3328740-3328363 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT190681 NZ_CP091523:2755672-2755842 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E2L1N0

Antitoxin

• AlphaFold DB: A0A5Z3CIS0