Detailed information of TA system TA190675

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 906224..907055
• Replicon: chromosome
• Accession: NZ_CP069500
• Organism: Escherichia coli strain FDAARGOS_1258

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: I6J88_RS04995
• Protein ID: WP_204086150.1
• Coordinates: 906681..907055 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1PWQ3
• Locus tag: I6J88_RS04990
• Protein ID: WP_001285586.1
• Coordinates: 906224..906592 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J88_RS04955	901441..902511	+	1071	WP_096970238.1	patatin-like phospholipase family protein	-
I6J88_RS04960	902647..903324	+	678	WP_001362823.1	hypothetical protein	-
I6J88_RS04965	903340..903750	+	411	WP_000846704.1	hypothetical protein	-
I6J88_RS04970	903971..904792	+	822	WP_001234569.1	DUF945 domain-containing protein	-
I6J88_RS04975	904883..905368	+	486	WP_000213703.1	antirestriction protein	-
I6J88_RS04980	905384..905860	+	477	WP_001351157.1	RadC family protein	-
I6J88_RS04985	905929..906150	+	222	WP_000692323.1	DUF987 domain-containing protein	-
I6J88_RS04990	906224..906592	+	369	WP_001285586.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
I6J88_RS04995	906681..907055	+	375	WP_204086150.1	TA system toxin CbtA family protein	Toxin
I6J88_RS05000	907052..907246	+	195	WP_000988601.1	hypothetical protein	-
I6J88_RS05005	907259..907372	+	114	WP_001161660.1	DUF957 domain-containing protein	-
I6J88_RS05010	907661..907807	-	147	Protein_854	transposase domain-containing protein	-
I6J88_RS05015	907861..908043	+	183	WP_001016348.1	hypothetical protein	-
I6J88_RS05020	908144..908473	-	330	WP_000450409.1	DUF496 family protein	-
I6J88_RS05025	908645..909703	-	1059	WP_001200890.1	FUSC family protein	-
I6J88_RS05030	909901..910374	-	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
I6J88_RS05035	910493..911659	-	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13928.97 Da
• Isoelectric Point: 7.1326

>T190675 WP_204086150.1 NZ_CP069500:906681-907055 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILWARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T190675 NZ_CP091523:1234914-1235102 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13563.41 Da
• Isoelectric Point: 5.0468

>AT190675 WP_001285586.1 NZ_CP069500:906224-906592 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT190675 NZ_CP091523:1234625-1234885 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Antitoxin

• AlphaFold DB: S1PWQ3