Detailed information of TA system TA190666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 328946..330534
• Replicon: chromosome
• Accession: NZ_CP069500
• Organism: Escherichia coli strain FDAARGOS_1258

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: I6J88_RS02255
• Protein ID: WP_001125475.1
• Coordinates: 328946..330268 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6J88_RS02260
• Protein ID: WP_001332145.1
• Coordinates: 330268..330534 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J88_RS02225	323957..324739	-	783	WP_000699097.1	SDR family oxidoreductase	-
I6J88_RS02230	325037..325348	+	312	WP_001468159.1	transcriptional regulator	-
I6J88_RS02235	325597..326661	-	1065	WP_000859696.1	alpha/beta fold hydrolase	-
I6J88_RS02240	326819..327580	-	762	WP_001066622.1	carboxymuconolactone decarboxylase family protein	-
I6J88_RS02245	327558..328037	-	480	WP_015912499.1	cupin domain-containing protein	-
I6J88_RS02250	328026..328687	+	662	Protein_314	alpha/beta hydrolase	-
I6J88_RS02255	328946..330268	-	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6J88_RS02260	330268..330534	-	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6J88_RS02265	330842..331600	+	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
I6J88_RS02270	331604..332518	-	915	WP_001306069.1	bestrophin family protein	-
I6J88_RS02275	332715..334166	-	1452	WP_000854636.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T190666 WP_001125475.1 NZ_CP069500:c330268-328946 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T190666 NZ_CP091522:2144048-2144302 [Neisseria sp. Dent CA1/247]
ATGAAAAAGAAACACCAAAGAACGCTTGCTTTAATATTCAGCCGCCCCGTATCCGGCAGCATTAAATGGGCGGATATTGA
AGCCCTGTTTGTTGAATTGGGCGCGGAAATACAAGAAAGAGAAGGCAGCCGTGTGGCCGTAATCCTTTTCGGGCAAGTGA
AAGTGTTTCATAGGCCGCATCCAAGCCCTGATACGGATAAAGGCGCGGTTGCGAGCGTGCGTAAATGGTTGGAAGAAAAC
GGAGTAAAGCCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT190666 WP_001332145.1 NZ_CP069500:c330534-330268 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT190666 NZ_CP091522:2144299-2144634 [Neisseria sp. Dent CA1/247]
ATGAACAATGTAATGGAAATCAACGGCCATAAAGCCATTATTCAATACGACCCTGAAACCGAGTTGCTGCGCGGCGAGTT
TGTCGGCCTTAACGGCGGGGCGGATTTTTACGCCGATAACGTGGCAGACTTGCACAAAGAAGGGGTGGCCAGTTTGGCGG
TTTTTCTCGATGTGTGCAAAGAAAAAGGCATTGAGCCGTATAAACAGTATTCAGGCCGCTTTAATGTGCGGGTAAGCCCT
GAAACCCATGCAGCGGCGGCAGCGGCGGCAGCGGCGCAAAATATCAGCCTGAATGAATGGGTAAACCGTGCAATCAGCCA
ATCCGCCCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8