Detailed information of TA system TA190662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 109092..109651
• Replicon: plasmid unnamed
• Accession: NZ_CP069499
• Organism: Escherichia coli strain FDAARGOS_1258

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A376ZSB3
• Locus tag: I6J88_RS00580
• Protein ID: WP_021515398.1
• Coordinates: 109092..109367 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6J88_RS00585
• Protein ID: WP_024187480.1
• Coordinates: 109367..109651 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J88_RS00565	106396..107172	-	777	WP_021515395.1	energy transducer TonB	-
I6J88_RS00570	107180..108055	-	876	WP_021515396.1	ChaN family lipoprotein	-
I6J88_RS00575	108332..108826	+	495	Protein_114	IS91 family transposase	-
I6J88_RS00580	109092..109367	-	276	WP_021515398.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J88_RS00585	109367..109651	-	285	WP_024187480.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6J88_RS00590	110199..110627	-	429	WP_021515400.1	Exc2 family lipoprotein	-
I6J88_RS00595	110708..110887	-	180	Protein_118	entry exclusion protein 1	-
I6J88_RS00600	111079..111726	+	648	WP_021515401.1	hypothetical protein	-
I6J88_RS00605	111951..112250	-	300	WP_021515402.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6J88_RS00610	112240..112503	-	264	WP_001089474.1	hypothetical protein	-
I6J88_RS00615	112610..112870	-	261	WP_019842130.1	hypothetical protein	-
I6J88_RS00620	113573..114430	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
I6J88_RS00625	114423..114497	-	75	WP_001365705.1	RepA leader peptide Tap	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..118909	118909
	-	flank	IS/Tn	-	-	108338..108826	488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10778.55 Da
• Isoelectric Point: 10.2344

>T190662 WP_021515398.1 NZ_CP069499:c109367-109092 [Escherichia coli]
MELKWTSKALSNLARLYDFLVLASKPAAARMVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T190662 NZ_CP091519:c1262978-1262568 [Alysiella crassa]
ATGATTTATCTGTTGGATACCAATATGATAGTGTATTTAATGCGAAATCGCCCCGAAATCGTGGCGCGTAAAATCGCCAG
TTTACCGCGCCACGATAAAGTGTGCATGAGTTTTATCACCTACAGTGAATTGCTCAAAGGCGTATATCGCAGCGAATTAT
CTGAAAAATCAAAGCACAAACTAGAACTGTTGGCAAAACATATTGATGTATTATTGCCACGCGAAGCCATCAGCGAACAT
TATGCCCGTTTGGGCGCACAACTGAAACAACAAGGCACACCCATCGGCAATAATGACTTGTGGATTGCCGCCCACGCACG
CTCACTTGGTGCAGTGCTGGTGAGCAATCAAAGCCAAATTTTTCAAAATATTAGCGGTTTAATCATTGAAGATTGGACAC
AGGCAGCCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10470.89 Da
• Isoelectric Point: 5.6558

>AT190662 WP_024187480.1 NZ_CP069499:c109651-109367 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT190662 NZ_CP091519:c1263217-1262975 [Alysiella crassa]
ATGCTCACCAGAGTCTTTCAAAGCGGCAACAGCCAAGCGGTGCGTATTCCCATCAGTTTCCGTTTTAAGGTGGACACGGT
GGAAATTTTACACGGCGACAATGGCGATGTGATTTTACGCCCCATTCAACCCGATACACGCGGCGAAGATTTTTTAGCGT
TATTTGAAGGATTTGACGAAGACTTTATCCGTGCGGTGGAAGAACGCGATAATACACCACCACAGGAGCGCGATGCATTA
TGA

Structures

Toxin

• AlphaFold DB: A0A376ZSB3