Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 59584..60109 Replicon plasmid unnamed
Accession NZ_CP069499
Organism Escherichia coli strain FDAARGOS_1258

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag I6J88_RS00385 Protein ID WP_001159871.1
Coordinates 59584..59889 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6J88_RS00390 Protein ID WP_000813634.1
Coordinates 59891..60109 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J88_RS00355 54851..56091 + 1241 Protein_70 translesion error-prone DNA polymerase V subunit UmuC -
I6J88_RS00360 56126..56518 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
I6J88_RS00365 56523..57494 - 972 WP_021515435.1 stable plasmid inheritance protein -
I6J88_RS00370 57723..58367 + 645 WP_000633911.1 AAA family ATPase -
I6J88_RS00375 58361..58636 + 276 WP_000239529.1 hypothetical protein -
I6J88_RS00380 58774..59583 - 810 WP_021515436.1 site-specific integrase -
I6J88_RS00385 59584..59889 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
I6J88_RS00390 59891..60109 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6J88_RS00395 62430..63071 + 642 WP_021515437.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..118909 118909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T190661 WP_001159871.1 NZ_CP069499:c59889-59584 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T190661 NZ_CP091519:1065931-1066272 [Alysiella crassa]
TTGGCGAGTATTTTCAATGCAGAATGGGACGAATTTAGCGCAAAATCCAACCGCGAACAAATTGTCATTAGCCCCTACGC
CGATTTCCAAAATGAACTCATGGAAAATCCCGAAAAAGGCGATTTAATTCAAGGTACAGGCGGATTGAGAAAAATCCGTG
TTGCCGACCCCAAATGTCAAAAAGGCAAGCGTGGTGGTTCACGGGTGATTTATTATTGGCATTTAAATCAAGCTCAATTT
GTTTTGTTTACCGTTTATGGCAAAAATCAACAGGACGATTTAAACCATGAACAACGCCTGTTGGCGCAACTTCTTCAAGT
AATCAAGGACAATCAACCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT190661 WP_000813634.1 NZ_CP069499:c60109-59891 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT190661 NZ_CP091519:1066380-1066583 [Alysiella crassa]
GTGGTCATCAGCCCCGATGAAATTCGCGCCATTAGGGAGCAGTTGAATTTATCGCAAGCGGTGTTTGCACAAAAATTACG
TATGTCTGTGCGGACGTATCAGGGTTGGGAACAGGGTAAAACACGCCCAAATTCGCAGGCTGCTTTGTTGCTGAAAATGG
TAGCTCAATCGCCGCAAACTTTTCACAATATTGCCGCCATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References