Detailed information of TA system TA190658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 107552..108136
• Replicon: plasmid unnamed2
• Accession: NZ_CP069498
• Organism: Acinetobacter pittii strain FDAARGOS_1217

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N9JY46
• Locus tag: I6J47_RS18980
• Protein ID: WP_000286963.1
• Coordinates: 107552..107854 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: I6J47_RS18985
• Protein ID: WP_000985610.1
• Coordinates: 107855..108136 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J47_RS18950	102932..103102	+	171	Protein_113	putative addiction module antidote protein	-
I6J47_RS18955	103305..104513	+	1209	WP_000343018.1	IS256-like element ISAba26 family transposase	-
I6J47_RS18960	104517..104834	+	318	WP_009504781.1	hypothetical protein	-
I6J47_RS18965	104841..105263	-	423	WP_009504779.1	hypothetical protein	-
I6J47_RS18970	105900..106682	-	783	WP_006582115.1	hypothetical protein	-
I6J47_RS18975	106690..107352	-	663	WP_009504778.1	hypothetical protein	-
I6J47_RS18980	107552..107854	+	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J47_RS18985	107855..108136	+	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
I6J47_RS18990	108364..109224	+	861	WP_002009063.1	IS5 family transposase	-
I6J47_RS18995	109361..109732	-	372	WP_009501156.1	RcnB family protein	-
I6J47_RS19000	110069..110299	+	231	WP_009501150.1	hypothetical protein	-
I6J47_RS19005	110469..111005	-	537	WP_009501148.1	porin family protein	-
I6J47_RS19010	111713..112069	+	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6J47_RS19015	112062..112364	+	303	WP_001140619.1	XRE family transcriptional regulator	-
I6J47_RS19020	112357..112566	+	210	WP_000069471.1	hypothetical protein	-
I6J47_RS19025	112691..112903	-	213	WP_016653394.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..128321	128321
	-	inside	IScluster/Tn	-	-	93238..114144	20906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11545.02 Da
• Isoelectric Point: 4.6838

>T190658 WP_000286963.1 NZ_CP069498:107552-107854 [Acinetobacter pittii]
MYSIYTTEAFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T190658 NZ_CP091518:c1185114-1184836 [Kingella kingae]
ATAAATTACCGCTTTGTTGCCGAAGCGGAAGATGAATGGCGCAAGCTAGACAACAGCATCAAGCAACAATTTAAATCCGC
GCTAGCCAAACGCTTGGAAAATCCGCGTATTCCATCTGCTGCGTTACATAATATGCCTGATTGCTACAAAATTAAATTGC
GTAAAATCGGCTATCGTCTCGTTTATCGTGTTGATGATAATGTGCTTACGGTTACAGTCATCGCCATTGGTAAACGCGAT
AAAAGCGTAGTTTATGATGTGGCAAATAGTCGCTTGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT190658 WP_000985610.1 NZ_CP069498:107855-108136 [Acinetobacter pittii]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT190658 NZ_CP091518:c1185353-1185111 [Kingella kingae]
ATGCAAGCCGTATTAGCCGATTACGCCGTGAGCGTTGCCGAATTAAAGCGCAATTACGCTTCTATTATTGACGAAGCCAC
AGGCGCGGTTGCTGTGTTGAATCACAACAAGCTCGAAGCGTATTTAATCCCAGCGCAGCATTACGAGCAGTTAATGGATT
ATTTGGAAGATTTGCAAGACGCGCAAATCATTCAAGAGCGTATTAACTCACCAAGCCGCGAGGTTTCGCTAGATGAGTTA
TAA

Structures

Toxin

• AlphaFold DB: A0A836MGN2