Detailed information of TA system TA190654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2135395..2136052
• Replicon: chromosome
• Accession: NZ_CP069495
• Organism: Kytococcus sedentarius strain FDAARGOS_1200

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6J30_RS10075
• Protein ID: WP_198479761.1
• Coordinates: 2135645..2136052 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C7NG62
• Locus tag: I6J30_RS10070
• Protein ID: WP_015780397.1
• Coordinates: 2135395..2135625 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J30_RS10055	2130539..2130793	-	255	WP_041290961.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
I6J30_RS10060	2130867..2133749	-	2883	WP_204159701.1	type I DNA topoisomerase	-
I6J30_RS10065	2134008..2135201	+	1194	WP_204159702.1	Fic family protein	-
I6J30_RS10070	2135395..2135625	+	231	WP_015780397.1	hypothetical protein	Antitoxin
I6J30_RS10075	2135645..2136052	+	408	WP_198479761.1	PIN domain-containing protein	Toxin
I6J30_RS10080	2136256..2136789	+	534	WP_204159703.1	hypothetical protein	-
I6J30_RS10085	2136847..2138472	-	1626	WP_204159704.1	class I SAM-dependent methyltransferase	-
I6J30_RS10090	2138483..2140528	-	2046	WP_204159705.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15039.91 Da
• Isoelectric Point: 6.7619

>T190654 WP_198479761.1 NZ_CP069495:2135645-2136052 [Kytococcus sedentarius]
VNTLIALLDIDHRDHEAAHRWAEERLSNGWATCATTQMGFLRVISQPGYPSPITVDEAGRLLRRATDHPSHEYWSSEPQP
CAGSVSLDVITGHRQITDAYLLALAVHQNGCLVTFDRRITTRHVPGAGQEHLVRL

Nucleotide

• Length: 408 bp

>T190654 NZ_CP091516:1998331-1998516 [Kingella potus]
ATGAAAAGCAGGGAGTTAATCGCTATTCTCGAAGCCGGCGGCTGGCAGCTTGTCCGAGTGAAAGGCAGCCACCATCAGTT
TAAGAAAGACGGCGTTCCACACTTGATTACCATCAGCCATCCCGAAAAAGACGTATCTTTGGGGCAACTGAAAGATGCAA
AAAAGAAATCGGGTTTGGATTTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8195.17 Da
• Isoelectric Point: 4.5535

>AT190654 WP_015780397.1 NZ_CP069495:2135395-2135625 [Kytococcus sedentarius]
MRTTLTIEDDVLQAVKDRARARGTTAGQELSALARAALSAPTFVDVGERNGLPVLQHRGEPVTREQVEALLDEDPA

Nucleotide

• Length: 231 bp

>AT190654 NZ_CP091516:1998570-1998977 [Kingella potus]
ATGATTTACTATCCGGCCGCCCTGTTTCAAGATGCGGGGCAAAGCGGCTACGGCATAGTCATTCCCGACCTGCCCGGCTG
CTATCCTGTGGGCGATACGGTGGAGGAGGCCTTGGCCGACGCGAAAGCCGCCGCCGCGTTCCACATTGAGGGCATGATTG
CCGAGAGCTTGGAATATGTTACCGCGCCGCGCGGCATTGCCGAGCTGCGCAGCCTGCCCGATTATGCCGACGCGCTTATG
TGGGTGTTGGTAGAGGTGGATGAAACCGTGTTTTCCAAACAGACGCGCTTCAATGTGAGCTGGCCGCAGTATTTGCTCGA
CCGCGTGGACGAATACACCGCCATACACCATGAAACACGCAGCGGCTTTCTCGCCCGTGCCGCGCTGGACGCGCTCAACC
GTTCCTGA

Structures

Antitoxin

• AlphaFold DB: C7NG62