Detailed information of TA system TA190650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4894763..4895563
• Replicon: chromosome
• Accession: NZ_CP069488
• Organism: Escherichia coli strain FDAARGOS_1264

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: I6J94_RS23635
• Protein ID: WP_000342452.1
• Coordinates: 4895036..4895563 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: I6J94_RS23630
• Protein ID: WP_001277107.1
• Coordinates: 4894763..4895029 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J94_RS23610	4890422..4891090	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
I6J94_RS23615	4891083..4892141	+	1059	WP_001042013.1	permease-like cell division protein FtsX	-
I6J94_RS23620	4892386..4893240	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
I6J94_RS23625	4893511..4894614	+	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
I6J94_RS23630	4894763..4895029	+	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
I6J94_RS23635	4895036..4895563	+	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
I6J94_RS23640	4895560..4895943	-	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
I6J94_RS23645	4896366..4897475	+	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
I6J94_RS23650	4897523..4898449	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
I6J94_RS23655	4898446..4899723	+	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
I6J94_RS23660	4899720..4900487	+	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T190650 WP_000342452.1 NZ_CP069488:4895036-4895563 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T190650 NZ_CP091515:345531-345713 [Snodgrassella alvi wkB2]
ATGCATAGCTCAAAGGTTATTAAAAGGCTGCTTGATGATGGGTGGTATGAGGTAGCCGTTAGAGGCAGCCATCATCAATT
CAAACATCCGGAAAAATCAGGTCGTGTTACCGTCCCTCACCCTAAAAAGGATTTACCACTCGGGACAGTAAAAAATATAT
TTAAACAAGCCGGTCTTAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT190650 WP_001277107.1 NZ_CP069488:4894763-4895029 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT190650 NZ_CP091515:345745-346170 [Snodgrassella alvi wkB2]
ATGTTTCTCTACATTGCTATACACAAAGATGAAAACACCGGTTACGGTGTAACAGTGCCCTCTTTACCCGGCTGCTTTTC
ATATGGTGATACTTTAGAAAAGGCTATTGAAGAATCTAAACAAGCCATCTTATTTCATATCGAGGGTTTACTTGAAGATG
GCATAGAACCTGAAACAAATCAGCCCGACCTTGCCACTCTCATTGATACCCCTGAGTATGCAGGAGCACAATGGTTTGGT
ATCGAGGTGAATATTGATCACCTAACCCTTAAACCAGAACGATTTAATGTTAGCTGGCCTAAATACCTTTTGAACAAGGT
TGATACCTACGTATCACTAACCCACGATACCCGTTCTAACTTTCTTGCTAAAGCCGCACTTGAGAGAATTAATAGTGCCA
ATCAAAACGCCGGCATAGCTAAATGA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57