Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4066955..4067452 Replicon chromosome
Accession NZ_CP069488
Organism Escherichia coli strain FDAARGOS_1264

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6J94_RS19895 Protein ID WP_001173343.1
Coordinates 4066955..4067128 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6J94_RS19900 Protein ID WP_001312331.1
Coordinates 4067156..4067452 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J94_RS19885 4063625..4065082 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
I6J94_RS19890 4065319..4066836 + 1518 WP_000003806.1 lysine--tRNA ligase -
I6J94_RS19895 4066955..4067128 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6J94_RS19900 4067156..4067452 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6J94_RS19905 4067680..4067952 - 273 WP_000405647.1 N-acetyltransferase -
I6J94_RS19910 4068166..4072095 - 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4058584..4083043 24459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T190648 WP_001173343.1 NZ_CP069488:c4067128-4066955 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T190648 NZ_CP091512:c1325878-1325624 [Vitreoscilla stercoraria]
ATGAAACGAAAACATCAAAAAACCTTAGAACTGATTTTCCATCGACCAGTATCAGGAAGTATAAAATGGACGGAGATCGA
AGCCTTATTTATTGAGCTAGGAGCAAACGTGCAGGAGCGTGAAGGTTCACGTATAGCGGTTGTGTTATTCGGACAGGTAA
AAGTGTTTCATCGTCCGCATCCTAGCCCTGATACCGATAAAGGGGCTGTTGCCAGTGTTCGAAAGTGGTTAGATGAAAAT
GGAGTAAGACCATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT190648 WP_001312331.1 NZ_CP069488:c4067452-4067156 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT190648 NZ_CP091512:c1325627-1325292 [Vitreoscilla stercoraria]
ATGATTAATACGATGATAATTAATGGCTACAAAGCCGTGATTGCATATGATTCTGAAATTGAGTTGTTTCGCGGTGAATT
TATTGGATTAAATGGTGGAGCAGATTTTTATGCGGCTGATGTAGAAAGCTTACGTAGAGAAGGTGCTGAATCATTAAAAG
TGTTTTTAGAAGTGTGTAAAGAGAACGGTATCGAGCCAAAAAAACAATATTCAGGACGCTTTAATGTTCGGATTAATCCG
AAATTACATGAAGATGCCATGGCTGCTGCTATTGCTCAAGGCATCAGCCTAAATGAGTTAGTTAGCCGTGCCATTGCCAC
AGCAGCACATCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References