190644

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3782158..3782734 Replicon chromosome
Accession NZ_CP069488
Organism Escherichia coli strain FDAARGOS_1264

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag I6J94_RS18490 Protein ID WP_001295743.1
Coordinates 3782447..3782734 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag I6J94_RS18485 Protein ID WP_000063148.1
Coordinates 3782158..3782460 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J94_RS18470 3778754..3780904 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
I6J94_RS18475 3780954..3781157 + 204 WP_000467859.1 YbdD/YjiX family protein -
I6J94_RS18480 3781168..3782124 + 957 WP_001295745.1 GTPase -
I6J94_RS18485 3782158..3782460 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
I6J94_RS18490 3782447..3782734 - 288 WP_001295743.1 BrnT family toxin Toxin
I6J94_RS18495 3782931..3784097 + 1167 WP_000800831.1 restriction endonuclease -
I6J94_RS18500 3784161..3785780 + 1620 WP_001029745.1 type I restriction-modification system subunit M -
I6J94_RS18505 3785770..3787074 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T190644 WP_001295743.1 NZ_CP069488:c3782734-3782447 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T190644 NZ_CP091509:c1984969-1984709 [Neisseria dumasiana]
ATGAAACACCGCTTTACCGCAGATGCAGCAGAGGACTTAGCCTACTGGGTAAAGCATGATGGAAGGAAGGTGGAACGGAT
TAAAGTTCTGTTGGAAGATATTAAAATCAACCACCCTAAAGGCATCGGTAAGCCGGAGCCACTCAGGCATCAGAAATCAG
GTTTGTGGTCGCGTAGGATAGATCGGGAACACAGACTGGTTTACTCCGTAGAAGAAGATACGGTAACAGTTTATGCTTGT
CGCTTTCACTATGGACGTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT190644 WP_000063148.1 NZ_CP069488:c3782460-3782158 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT190644 NZ_CP091509:c1985214-1984966 [Neisseria dumasiana]
ATGGACAATGTAGTCAGTTACACCGCTGCTCGAGAAGACTTGGCCAACATCATGACCAAAGTGTGTGATGATCATGTCGT
TACACATATTACCCGCCGCAACGGTGGCAATTGTGTTTTGATGTCGGAAGAAGAGTACAACTCAATTATGGAAACGCTTT
ATCTGTTTGGCAACCCGACCAATGCTGCCCGTTTGGAGCATTCTTTGGCTCAAGCCGAGCGTGGCGAGTTTGTGGATGTC
GAATTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References