Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3217977..3218595 Replicon chromosome
Accession NZ_CP069488
Organism Escherichia coli strain FDAARGOS_1264

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6J94_RS15790 Protein ID WP_001291435.1
Coordinates 3218377..3218595 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6J94_RS15785 Protein ID WP_000344800.1
Coordinates 3217977..3218351 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J94_RS15775 3213066..3214259 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6J94_RS15780 3214282..3217431 + 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
I6J94_RS15785 3217977..3218351 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6J94_RS15790 3218377..3218595 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6J94_RS15795 3218769..3219320 + 552 WP_000102539.1 maltose O-acetyltransferase -
I6J94_RS15800 3219436..3219906 + 471 WP_000136192.1 YlaC family protein -
I6J94_RS15805 3220070..3221620 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6J94_RS15810 3221662..3222015 - 354 WP_000878135.1 DUF1428 family protein -
I6J94_RS15820 3222394..3222705 + 312 WP_000409908.1 MGMT family protein -
I6J94_RS15825 3222736..3223308 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T190641 WP_001291435.1 NZ_CP069488:3218377-3218595 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T190641 NZ_CP091508:c2127349-2127056 [Uruburuella testudinis]
ATGACTTATGAATTGGCTTTTCTGCCCTCTGCGCGGCGGGAATGGGACAAGCTCGACAACAGCATCAAAACCTTGTTCAA
ACGCAGGTTGGCAGAACGCTTGGAAAACCCGCATATGCCGTCTGCGGCCTTGCACGGCATGAGGAATGCTTACAAAATCA
AATTGCGCCAAGCCGGTTACCGTTTGGTTTACACGGTGGATGACGGCCTGATTACGGTAACGGTGGTGGCGGTGGGCAGA
CGTGATAAAAGCACGGTGTATGTACAGGCCTTGGCGCGGGCGAAGGATAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT190641 WP_000344800.1 NZ_CP069488:3217977-3218351 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT190641 NZ_CP091508:c2127581-2127339 [Uruburuella testudinis]
ATGGAAGCAGTATTGGCAGATTATGCTGTAAGCGTGACAGAGCTTAAGCGCAATTATGCAGCCATAATGGCGCAGGCAGA
TGGTGCCGTGGCGGTGCTGAACCACAACAAGCCTGAAGCCTATCTGATTTCTGCTGAGCGTTATCAGGCGCTGCTGGAGT
ATATGGAAGATTTGGAAGATGTAAAGCTGGCGCTGGAGCGTTCAGATGAACAGGCAGTGAAGGTGTCTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References