190638

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 1946400..1947001 Replicon chromosome
Accession NZ_CP069488
Organism Escherichia coli strain FDAARGOS_1264

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag I6J94_RS09405 Protein ID WP_001216034.1
Coordinates 1946621..1947001 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag I6J94_RS09400 Protein ID WP_001190712.1
Coordinates 1946400..1946621 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J94_RS09380 1942015..1943010 + 996 WP_000246636.1 hypothetical protein -
I6J94_RS09385 1943014..1943946 + 933 WP_000991832.1 hypothetical protein -
I6J94_RS09390 1944083..1944780 - 698 Protein_1833 IS1 family transposase -
I6J94_RS09395 1944814..1946217 - 1404 WP_204097961.1 type I restriction-modification system subunit M -
I6J94_RS09400 1946400..1946621 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I6J94_RS09405 1946621..1947001 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I6J94_RS09410 1947006..1947185 + 180 WP_001513661.1 hypothetical protein -
I6J94_RS09415 1947213..1947572 + 360 WP_001513660.1 hypothetical protein -
I6J94_RS09420 1947496..1947909 + 414 Protein_1839 DDE-type integrase/transposase/recombinase -
I6J94_RS09425 1947859..1948176 - 318 WP_001513659.1 hypothetical protein -
I6J94_RS09430 1948404..1949420 - 1017 WP_012372828.1 IS5-like element IS5 family transposase -
I6J94_RS09435 1949628..1951031 + 1404 WP_001373486.1 S-methylmethionine permease -
I6J94_RS09440 1951018..1951950 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1939057..1955192 16135
- inside IScluster/Tn - - 1939057..1953101 14044


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T190638 WP_001216034.1 NZ_CP069488:1946621-1947001 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T190638 NZ_CP091507:1447341-1447481 [Uruburuella suis]
ATGTTGAAAAAAACCGTATGGCTGGCCATGGCTGCCGCCGTTTTGCTGAGCGCCTGCAACACTGTTTCAGGCTTTGGCGA
AGATGTTCAAAAAGCCGGCAACAAGCTTGAGCAATCTGCCGACCGCCACGGCGCAAACTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT190638 WP_001190712.1 NZ_CP069488:1946400-1946621 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT190638 NZ_CP091507:1447191-1447313 [Uruburuella suis]
ATGAAAAAAATCATCTTGATCGGCTTGACCGCGATCACATTGTTATCCGCCTGCAACACCGTTTCCGGCTTTGGCAAAGA
TGTGCAGAAAGTCGGCGATAAAGTTGAAAAAGCAGCAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References