190637

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1940782..1941307 Replicon chromosome
Accession NZ_CP069488
Organism Escherichia coli strain FDAARGOS_1264

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag I6J94_RS09370 Protein ID WP_001159868.1
Coordinates 1940782..1941087 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6J94_RS09375 Protein ID WP_000813634.1
Coordinates 1941089..1941307 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J94_RS09345 1936380..1937192 + 813 WP_000587585.1 protein YdhT -
I6J94_RS09350 1937204..1938808 - 1605 WP_000848170.1 FAD-NAD(P)-binding protein -
I6J94_RS09355 1938934..1939047 - 114 Protein_1826 YdhR family protein -
I6J94_RS09360 1939057..1939754 - 698 Protein_1827 IS1 family transposase -
I6J94_RS09365 1939975..1940781 - 807 WP_000016982.1 site-specific integrase -
I6J94_RS09370 1940782..1941087 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
I6J94_RS09375 1941089..1941307 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6J94_RS09380 1942015..1943010 + 996 WP_000246636.1 hypothetical protein -
I6J94_RS09385 1943014..1943946 + 933 WP_000991832.1 hypothetical protein -
I6J94_RS09390 1944083..1944780 - 698 Protein_1833 IS1 family transposase -
I6J94_RS09395 1944814..1946217 - 1404 WP_204097961.1 type I restriction-modification system subunit M -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1939057..1955192 16135
- inside IScluster/Tn - - 1939057..1953101 14044


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T190637 WP_001159868.1 NZ_CP069488:c1941087-1940782 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T190637 NZ_CP091499:c37282-36977 [Enterobacter cloacae complex sp. ECL404]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT190637 WP_000813634.1 NZ_CP069488:c1941307-1941089 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT190637 NZ_CP091499:c37502-37284 [Enterobacter cloacae complex sp. ECL404]
GTGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGTTGCTCAAGGCATACGACGTAAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGCGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATCGAGGCTAACGGATCGTTTGCTGACGAAAACAGGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References