Detailed information of TA system TA190625

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 431272..432106
• Replicon: chromosome
• Accession: NZ_CP069488
• Organism: Escherichia coli strain FDAARGOS_1264

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: I6J94_RS02185
• Protein ID: WP_000854690.1
• Coordinates: 431272..431649 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: I6J94_RS02190
• Protein ID: WP_001305076.1
• Coordinates: 431738..432106 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J94_RS02155	427666..427821	-	156	WP_000729638.1	hypothetical protein	-
I6J94_RS02160	428253..429186	-	934	Protein_420	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
I6J94_RS02165	429179..429574	-	396	WP_000208384.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
I6J94_RS02170	429643..430488	-	846	WP_001529401.1	DUF4942 domain-containing protein	-
I6J94_RS02175	430573..430770	-	198	WP_000839293.1	DUF957 domain-containing protein	-
I6J94_RS02180	430787..431275	-	489	WP_000761699.1	hypothetical protein	-
I6J94_RS02185	431272..431649	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
I6J94_RS02190	431738..432106	-	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J94_RS02195	432156..432800	-	645	WP_000094916.1	hypothetical protein	-
I6J94_RS02200	432819..433040	-	222	WP_000692329.1	DUF987 domain-containing protein	-
I6J94_RS02205	433103..433579	-	477	WP_001186726.1	RadC family protein	-
I6J94_RS02210	433595..434080	-	486	WP_000849565.1	antirestriction protein	-
I6J94_RS02215	434135..434953	-	819	WP_001234620.1	DUF945 domain-containing protein	-
I6J94_RS02220	435054..435287	-	234	WP_000902034.1	DUF905 family protein	-
I6J94_RS02225	435366..435821	-	456	WP_000581502.1	hypothetical protein	-
I6J94_RS02230	435897..437024	-	1128	Protein_434	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / sat	411806..480318	68512
	-	flank	IS/Tn	-	-	427666..427821	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T190625 WP_000854690.1 NZ_CP069488:c431649-431272 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T190625 NZ_CP091493:232867-234192 [Enterobacter cloacae complex sp. ECL405]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT190625 WP_001305076.1 NZ_CP069488:c432106-431738 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT190625 NZ_CP091493:232569-232862 [Enterobacter cloacae complex sp. ECL405]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8