Detailed information of TA system TA190604

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4612752..4613583
• Replicon: chromosome
• Accession: NZ_CP069480
• Organism: Escherichia coli strain FDAARGOS_1257

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: I6J87_RS21965
• Protein ID: WP_000854814.1
• Coordinates: 4612752..4613126 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6J87_RS21970
• Protein ID: WP_024174331.1
• Coordinates: 4613215..4613583 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J87_RS21925	4608148..4609314	+	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
I6J87_RS21930	4609433..4609906	+	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
I6J87_RS21935	4610104..4611162	+	1059	WP_001200890.1	FUSC family protein	-
I6J87_RS21940	4611334..4611663	+	330	WP_000450409.1	DUF496 family protein	-
I6J87_RS21945	4611764..4611946	-	183	WP_001016348.1	hypothetical protein	-
I6J87_RS21950	4612000..4612146	+	147	Protein_4284	transposase domain-containing protein	-
I6J87_RS21955	4612435..4612548	-	114	WP_001161660.1	DUF957 domain-containing protein	-
I6J87_RS21960	4612561..4612755	-	195	WP_000988601.1	hypothetical protein	-
I6J87_RS21965	4612752..4613126	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
I6J87_RS21970	4613215..4613583	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J87_RS21975	4613657..4613878	-	222	WP_000692346.1	DUF987 domain-containing protein	-
I6J87_RS21980	4613947..4614423	-	477	WP_001541535.1	RadC family protein	-
I6J87_RS21985	4614439..4614912	-	474	WP_000855074.1	antirestriction protein	-
I6J87_RS21990	4615175..4615996	-	822	WP_001234571.1	DUF945 domain-containing protein	-
I6J87_RS21995	4616217..4616627	-	411	WP_000846704.1	hypothetical protein	-
I6J87_RS22000	4616643..4617320	-	678	WP_001362823.1	hypothetical protein	-
I6J87_RS22005	4617456..4618526	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T190604 WP_000854814.1 NZ_CP069480:c4613126-4612752 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T190604 NZ_CP091486:c3836674-3836264 [Enterobacter cloacae complex sp. ECL411]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACTCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCACCTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT190604 WP_024174331.1 NZ_CP069480:c4613583-4613215 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT190604 NZ_CP091486:c3836921-3836655 [Enterobacter cloacae complex sp. ECL411]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCCGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50