Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3551300..3551954 Replicon chromosome
Accession NZ_CP069480
Organism Escherichia coli strain FDAARGOS_1257

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag I6J87_RS17135 Protein ID WP_000244765.1
Coordinates 3551547..3551954 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6J87_RS17130 Protein ID WP_000354046.1
Coordinates 3551300..3551566 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J87_RS17110 3547388..3548821 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
I6J87_RS17115 3548866..3549177 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6J87_RS17120 3549341..3550000 + 660 WP_000250274.1 hemolysin III family protein -
I6J87_RS17125 3550077..3551057 - 981 WP_000886080.1 tRNA-modifying protein YgfZ -
I6J87_RS17130 3551300..3551566 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6J87_RS17135 3551547..3551954 + 408 WP_000244765.1 protein YgfX Toxin
I6J87_RS17140 3551994..3552515 - 522 WP_001055874.1 flavodoxin FldB -
I6J87_RS17145 3552627..3553523 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6J87_RS17150 3553548..3554258 + 711 WP_000715229.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6J87_RS17155 3554264..3555997 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T190601 WP_000244765.1 NZ_CP069480:3551547-3551954 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T190601 NZ_CP091486:c1081160-1080942 [Enterobacter cloacae complex sp. ECL411]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGTGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT190601 WP_000354046.1 NZ_CP069480:3551300-3551566 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT190601 NZ_CP091486:c1081563-1081189 [Enterobacter cloacae complex sp. ECL411]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References